Calu-3 人類肺腺癌細胞（胸水）
| ATCC® Number: | HTB-55™ |
|
|
| Designations: | Calu-3 |
|
| | Depositors: | J Fogh |
|
| | Biosafety Level: | 1 |
|
| | Shipped: | frozen |
|
| | Medium & Serum: | See Propagation |
|
| | Growth Properties: | adherent |
|
| | Organism: | Homo sapiens (human) |
|
| | Morphology: | epithelial
|
|
| | Source: | Organ: lung
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion |
|
| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | Calu-3 人類肺腺癌細胞（胸水） |
|
| | Restrictions: | The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439. |
|
| | Applications: | transfection host (Roche FuGENE® Transfection Reagents) |
|
| | Tumorigenic: | Yes |
|
| | Antigen Expression: | Blood Type A; Rh+ |
|
| | DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 12,14 D5S818: 11 D7S820: 10,11 THO1: 6,9.3 TPOX: 8 vWA: 16,17 |
|
| | Cytogenetic Analysis: | The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximay 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations. |
|
| | Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
|
| | Age: | 25 years |
|
| | Gender: | male |
|
| | Ethnicity: | Caucasian |
|
| | Comments: | The patient had received prior therapy with cytoxan, bleomycin and adriamycin. |
|
| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C |
|
| | Subculturing: | Protocol:Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
|
|
|
|
MLE-12 小鼠肺上皮細胞
saos-2 人成骨肉瘤細胞
MA104 非洲綠猴胚胎腎上皮細胞
ball-1 細胞
nci-h226 人肺鱗癌細胞
MCA38 小鼠結腸癌細胞
LTPA 小鼠胰腺癌細胞
NCI-H23 人非小細胞肺癌細胞
293.00 人胚腎細胞
293FT 人胚腎細胞-用于慢病毒包裝
293TN 人胚腎細胞--用于慢病毒包裝
293AV 人胚腎細胞-用于腺病毒包裝
293T 人胚腎細胞
208F 大鼠成纖維細胞
786-O 人腎透明細胞腺癌細胞
A172 人腦膠質(zhì)母細胞瘤細胞
A549 人肺癌細胞 細胞系購買
AGS 人胃癌細胞
BEL7402 人肝癌細胞系
AZ-521 人胃癌細胞
B16F0 小鼠黑色素瘤細胞
Bel-7402 人肝癌細胞
Bel-7404 人肝癌細胞
BGC-823 人胃癌細胞
C6 大鼠腦膠質(zhì)瘤細胞
SW480 人結腸腺癌細胞
CaCo-2 人結腸癌細胞
Cho-K1 倉鼠卵巢細胞亞株
COS-7 非洲綠猴SV40轉(zhuǎn)化的腎細胞
GES-1 人胃上皮細胞
HCT-8 人回盲腸癌細胞
HeLa
Hepa1-6 小鼠肝癌細胞
HepG2 人肝癌細胞
hFOB1.19 人永生化成骨細胞
HGC-27 人胃癌細胞
Hs 746T 人胃癌細胞
HT-29 人結腸癌細胞
KB 人口腔表皮樣癌細胞
LoVo 人結腸癌細胞
LLC-PK1 豬腎近曲小管上皮細胞
MCF-10A 人正常乳腺上皮細胞
MCF-7 人乳腺癌細胞
MDA-MB-231 人乳腺癌細胞
MGC80-3 人胃癌細胞
MKN-45 人胃癌細胞
NCI-H460 人大細胞肺癌細胞
NIH-3T3 小鼠成纖維細胞
NUGC-3 人胃癌細胞
Raw264-7 小鼠單核細胞白血病細胞
SGC-7901 人胃癌細胞
SMMC-7721 人肝癌細胞
SNU-638 人胃癌細胞
SNU-739 人肝癌細胞
Tca8113 人舌鱗癌細胞 細胞系購買
U251 人腦膠質(zhì)瘤細胞
U-373MG 人腦神經(jīng)膠質(zhì)瘤細胞
U-87 MG 人腦星形膠質(zhì)母細胞瘤
BT-474 人乳腺導管癌細胞
PANC-1 人胰腺癌細胞
HEK293 人胚腎細胞
Hep3B 人肝癌細胞
HL-7702 人正常肝細胞
Raw-Blue 小鼠單核細胞白血病細胞
WEHI-231 小鼠B淋巴細胞
OPM-2 人骨髓瘤細胞
NCI-H929 人骨髓瘤細胞
U266 人骨髓瘤細胞
EJ 人膀胱癌細胞
HOC 大鼠肝卵圓細胞
RWPE-1 人正常前列腺上皮細胞
CAL 27 人舌鱗癌細胞
Calu-3 人肺腺癌細胞
Eca-109 人食管癌細胞
DLD-1 人結直腸腺癌上皮細胞
5637.00 人膀胱癌細胞
SK-OV-3 人卵巢癌細胞
GBC-SD 人膽囊癌細胞
BEL7402 人肝癌細胞系
SW620 人結腸癌細胞
MRC-5 人胚肺成纖維細胞 細胞系購買
PC9 人肺癌細胞
PC14 人肺癌細胞
BxPC 人原位胰腺腺癌細胞
PK（15） 豬腎上皮細胞
MDCK 狗腎上皮細胞
3D4/21 豬肺泡巨噬細胞
MHCC-97L 人肝癌細胞（低轉(zhuǎn)移）
MHCC-97H 人肝癌細胞系（高轉(zhuǎn)移）
MiaCaPa-2 人胰腺癌細胞
HN13 口腔鱗狀細胞癌細胞
Bend3 小鼠腦微血管內(nèi)皮細胞
QBC939 人膽管癌細胞
Ishikawa 人子宮內(nèi)膜癌細胞
A498 人腎癌細胞
143B
WEHI-231 小鼠B淋巴細胞
H322 人非小細胞肺癌細胞
Panc O2
S8 BEL7402 人肝癌細胞系
R5 細胞系購買
Pig MSC 豬骨髓間質(zhì)干細胞
HCAEC 人冠狀動脈內(nèi)皮細胞
marc-145 猴胚胎腎上皮細胞
3D4/21 豬肺泡巨噬細胞
PK（15） 豬腎上皮細胞
MADB-106 大鼠乳腺癌細胞
Sf9 昆蟲卵巢細胞
DADY
HUVEC[HUV-EC-C] 人臍靜脈內(nèi)皮細胞
PC14 人肺癌細胞
PC9 人肺癌細胞
Eca-109 人食管癌細胞
CAL 27 人舌鱗癌細胞
HOC 肝卵圓細胞
EJ 人膀胱癌細胞
U266 人骨髓瘤細胞
293-mTLR5 人胚腎細胞
Raw-Blue 小鼠單核細胞白血病細胞
HL-7702 人正常肝細胞
Hep3B 人肝癌細胞
U-373MG 人腦神經(jīng)膠質(zhì)瘤細胞
Tca-8113 人舌鱗癌細胞
SNU-739 人肝癌細胞
SNU-638 人胃癌細胞
NUGC-3 人胃癌細胞
NIH-3T3 小鼠成纖維細胞
MKN-45 人胃癌細胞
LLC-PK1 豬腎近曲小管上皮細胞
Hs 746T 人胃癌細胞
Hepa1-6 小鼠肝癌細胞
HCT-8 人回盲腸癌細胞
BEL7402 人肝癌細胞系
HaCaT 人永生化表皮細胞
GES-1 人胃上皮細胞
B16-F0 小鼠黑色素瘤細胞
AZ-521 人胃癌細胞
786-O 人腎透明細胞腺癌細胞
208F 大鼠成纖維細胞
293TN 人胚腎細胞--用于慢病毒包裝
293FT 人胚腎細胞-用于慢病毒包裝
GP-293 人胚腎細胞
293-L.P. 人胚腎細胞
HTB-55 Calu-3 人類肺腺癌 （胸水）
產(chǎn)品型號：HTB-55
