CRL-1999 T/G HA-VSMC 人血管平滑肌細胞

ATCC® Number: CRL-1999™ Price: $264.00

Designations: T/G HA-VSMC

Depositors: RR Gifford

Bioped: frozen

Medium & Serum: See Propagation

Growth Propersafety Level: 1

Shipties: adherent

Organism: Homo sapiens （human）

Morphology: fibroblast

CRL-1999 T/G HA-VSMC 人血管平滑肌細胞

Source: Organ: aorta

Tissue: smooth muscle

Disease: normal

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Applons: tranicatisfection host （technology from amaxa）

Reverse Transcript: N

DNA Profile （STR）: Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 9,10

THO1: 7,8

TPOX: 8

vWA: 16

Cytogenetic Analysis: This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal.

Age: 11 months

Gender: female

Ethnicity: Caucasian

HeLa Markers: N

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement （ECGS）; fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004

recommended serum:ATCC 30-2020

CRL-1999 T/G HA-VSMC