CRL-1732 FOX-瘤細胞 的詳細介紹
CRL-1732 FOX-NY 小鼠淋NY 小鼠淋巴巴瘤細胞
| ATCC® Number: | CRL-1732™ | Price: | $438.00 |
| Designations: | FOX-NY |
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| | Depositors: | RT Taggart |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | suspension |
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| | Organism: | Mus musculus （mouse） |
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| | Morphology: | lymphoblast
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| | Source: | Disease: myeloma
Strain: BALB/c
Cell Type: B lymphoblast; |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | CRL-1732 |
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| | Comments: | FOX-NY was derived from the NS-1 myeloma cell line. It is resistant to analogs of both adenosine and guanine （e.g., 2,6-diaminopurine and 8-azaguanine） as a result of deficiencies in hypoxanthine phosphoribosyl transferase （HPRT, HGPRT） and adenosine phosphoribosyl transferase （APRT）. When used as the immortal partner in a fusion using B cells from a female mouse with the Rb（8.12） translocation, there is a positive selection for the immunoglobulin heavy chain locus. If the fusion products are grown in the presence of aminopterin, adenine and thymidine （or azaserine and adenine）. Selection for the Igh locus results in more stable antibody production in the resulting hybridomas. Tested and found negative for ectromelia virus （mousepox）. |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
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