TIB-146 RA3 3A1/6.1 大鼠X小鼠雜合脾，骨髓瘤細胞， ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和優培養條件
TIB-146 RA3 3A1/6.1 大鼠X小鼠雜合脾，骨髓瘤細胞 的詳細介紹
TIB-146
| ATCC® Number: | TIB-146™ | Price: | $399.00 |
| Designations: | RA3-3A1/6.1 |
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| | Isotype: | IgM |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | suspension |
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| | Organism: | Rattus norvegicus （B cell）; Mus musculus （myeloma） （rat （B cell）; mouse （myeloma）） |
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| | Morphology: | lymphoblast
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| | Source: | Cell Type: hybridoma: B lymphocyte; |
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| | Cellular Products: | immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen （200000 dalton form of the T200 glycoprotein, CD45R） |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | Comments: | Animals were immunized with the mouse cell line RAW 112. Spleen cells were fused with the S194/5.XX0.BU.1 myeloma. Tested and found negative for ectromelia virus （mousepox）. |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| | Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml, and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml. |
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| | References: | 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164 927: Coffman RL, Weissman IL. B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340 32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902 |
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